Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
نویسندگان
چکیده
منابع مشابه
Live Cell Imaging in Fission Yeast.
Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic compositio...
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The development of cloning vectors for green fluorescent protein (GFP) and the simplicity of yeast reverse genetics allow straightforward labeling of yeast proteins in living cells. Budding and fission yeast are therefore attractive organisms in which to study dynamic cellular processes such as growth, cell division, and morphogenesis using live cell fluorescence microscopy. This article focuse...
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ژورنال
عنوان ژورنال: STAR Protocols
سال: 2020
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2020.100160